Lab and Research Technician 2 employees provide increasingly complex technical support for projects and may engage in increasingly complex scientific experiments. May handle more sophisticated administrative and logistical tasks. Lab and Research Technician 2 employees operate under supervision with a degree of independence when following set protocols and procedures.
The Schulman Lab in the Department of Pharmacology at the University of Virginia seeks a Laboratory and Research Technician 2 to assist investigators with experiments examining the regulation of transcription by members of the nuclear hormone receptor superfamily and exploring links between lipid metabolism and inflammation in several model systems. The individual will conduct complex research activities and make decisions according to ethical standards, understand laboratory processes and procedures, and perform minor data analysis. Further, the incumbent will have excellent written and communication skills, strong organizational and interpersonal skills, the ability to follow instructions, and general laboratory knowledge (i.e., lab procedures, basic safety precautions, how to handle samples, etc.). Familiarity with basic laboratory equipment (i.e., centrifuges, pH meters, pipettors) and the ability to perform scientific calculations is expected.
What You Will Do:
Experimental Work: Isolate RNA. Quantify gene expression by QPCR. Maintain cells in culture. Perform chromatin immunoprecipitation experiments. Work with mice, including maintaining mouse colony and genotyping mutant mice. Make protein extracts and perform Western blotting. Make libraries for next-generation sequencing. Perform flow cytometry experiments. Examine tissues by immunohistochemistry. Operate basic laboratory equipment, including centrifuges, PCR thermocyclers, power sources and spectrophotometers.
Laboratory Maintenance and General Duties: Maintain a clean laboratory area and assist with overall laboratory maintenance. Make buffers and other reagents. Maintain a laboratory notebook. Be available to assist principal investigators as needed.
Ordering and Purchasing: Process purchases for the lab using the UVA Marketplace. Interact with vendors to determine appropriate products. Return damaged or incorrect items.
Learn more about the Schulman Lab at https://pharm.virginia.edu/faculty/primary-faculty/?facbio=1&id=25996.
Associate's degree in biology, chemistry, molecular biology, biochemistry or a related discipline.
At least 2 years of lab experience (which may include student experience). A bachelor's degree may substitute for experience.
Experience with a variety of cellular, molecular and biochemical techniques.
Ability to synthesize information, provide advice and address needs independently.
Knowledge of general lab procedures, basic safety precautions and lab equipment.
Ability to work independently and as part of a team.
Experience with Microsoft Office (Word, PowerPoint, and Excel) and statistical/bioinformatics analyses.
Preference will be given to applicants with a bachelor's degree in a scientific discipline, laboratory experience working with live animals in an academic biomedical research environment, and knowledge of GraphPad and/or other statistical software.
This position is restricted and contingent upon the continuation of funding.
Anticipated Hiring Rage: $33,000 - 38,000 annually ($15.86 - $18.27/hour)
Salary will be commensurate with education, skills, and experience.
The selected candidate will be required to complete a background check prior to their first day of employment and a health screening per University policy.
Review of applicants will begin February 4, 2022, and the position will remain open until filled.
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