Lab and Research Technician 2, Department of Surgery
Job ID: R0034786 | Laboratory Research | Full Time | Charlottesville, VA
The Department of Surgery at the University of Virginia School of Medicine seeks a Lab and Research Technician 2 to support research focused on vascular biology and physiology under the supervision of Dr. Bowen Wang, Assistant Professor of Surgery. The primary goal of the research team is to determine the therapeutic and mechanistic implications of dietary restrictions in vascular cell phenotype changes (e.g., smooth muscle and endothelial cells) and vascular diseases (e.g., aneurysm, restenosis, thrombosis). The team also investigates both the upstream and downstream regulatory mechanisms that involve the following two emerging areas: protein post-translational modification (PTM) and gut microbiome.

Position Responsibilities

Laboratory Duties:
Experience working independently in a cell/molecular biology laboratory is preferred, but not required. Individual will independently perform various cell, molecular and biochemical procedures such as RNA/DNA purification, protein assays, Western blot, agarose and polyacrylamide gel electrophoresis, polymerase chain reaction (PCR) and ELISAs. Experience with microscopy and the use of antibodies in immunohistochemistry, flow cytometry and confocal microscopy is preferred, but not required. Routine laboratory duties include maintenance, organization and inventory of all laboratory equipment and supplies.

Team Environment: Individual should have experience working as part of a diverse team comprising students, postdoctoral researchers and principal investigators.

Animal Work: Individual should have experience with rodent handling and mouse colony maintenance, including inventory, breeding, genotyping and postoperative care. Individual will independently maintain transgenic mouse colonies (>4 strains) and assist with surgical preparations for rodents, including animal prep, surgical procedures, treatments, euthanasia and tissue collection.

Cell Culture: Experience with cell and tissue culture is not required, but preferred. Individual will independently perform cell culture studies, including experimental design and analysis, as well as maintenance of frozen cells. These studies will involve the use of human and mouse cell lines, as well as isolation of primary cells from human or mouse samples.

Computer: Individual should have experience with the use of Microsoft Word, Excel and PowerPoint in the laboratory setting.

This is a restricted position; continuation is dependent on funding and satisfactory performance. Review of applications will begin on April 12, 2022, but the position will remain open until filled.

Minimum Requirements:
  • Education: Associate's degree.
  • Experience: At least 2 years of lab experience, which may include student experience. Bachelor's degree in related science will be considered in lieu of experience.

Physical Demands:
This position can spend extensive periods of time standing and squatting while conducting experiments or caring for animals. Also requires keyboarding and other bench work and animal procedures requiring fine finger manipulation.

The successful candidates will pass a criminal background check and pre-employment health screening.

For questions about the position, please contact Dr. Bowen Wang, Assistant Professor of Surgery, at

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Charlottesville, VA
Charlottesville, VA 22903

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