The Department of Pathology at the University of Virginia is seeking a Lab and Research Tech 2 to join the lab of Dr. Mani Mahadevan. The Mahadevan lab uses mouse models to study RNA toxicity and its role in myotonic muscular dystrophy disease pathogenesis with a hope to find a treatment for this neuromuscular disease. The lab tech 2 will work closely with the PI and other lab members, as well as independently, to complete experiments. Preference will be given to applicants with experience in working with mice, PCR genotyping, protein isolation and immunoblotting, tissue sectioning, tissue staining, and immunohistochemistry.
Job Responsibilities Include:
Laboratory Maintenance and General Duties:
Maintain transgenic mouse colony (genotyping, husbandry, mouse ordering, interacting with vet techs, database management). Learn and perform a variety of experimental procedures, including, but not limited to, phenotyping the mice, tissue harvesting and preparation, anesthesia administration, tissue sectioning and immunohistochemistry, protein isolation, immunoprecipitation, and western blotting.
Keep track of drug inventories for sedatives and anesthetics and maintain detailed logs of usage and disposal of controlled substances.
Assist with data analysis. Maintain a detailed laboratory notebook.
Engage in laboratory research by performing complex lab and research-related tasks.
Develop, design, and conduct increasingly complex research projects. Perform complex tasks independently, adapting methods and procedures to meet the needs of research.
Maintain a clean laboratory area, lab equipment, and adequate inventory of lab supplies. Order supplies and reagents and interact with vendors and department fiscal specialists to keep track of invoices, orders, and budgets (for mice and supplies).
Make buffers and other reagents. Assist the PI with compliance for ACUC (mouse), IBC (biosafety), and chemical and environmental health safety protocols.
In addition to the above job responsibilities, other duties may be assigned.
This is a restricted position; continuation is dependent on funding and satisfactory performance. Priority review of applications will begin on December 17, 2022, but the position will remain open until filled.
Education: Associate's degree.
Experience: At least 2 years of lab experience, which may include student experience. Bachelor's degree in related science will be considered in lieu of experience.
Experience: Preference for experience managing a mouse colony, individuals with lab experience and ordering experience at UVA, and experience in wet lab research.
This position can require extensive periods of time standing and squatting while conducting experiments or caring for animals. Also requires keyboarding and other bench work and animal procedures requiring fine finger manipulation. The qualified candidate will have excellent work ethic, strong communication skills, and the ability to work both independently and collaboratively. The incumbent should also be self-motivated and meticulous and possess strong attention to detail.
The University of Virginia, established in 1819 by Thomas Jefferson, is recognized annually as one of the nation's top public institutions and the University of Virginia Medical Center is listed as one of America's top hospitals. Charlottesville is located in Central Virginia, 110 miles from Washington, D.C., and 70 miles from Richmond. It has been recognized as one of America's top cities to live, raise a family, conduct business, and retire. It has also been recognized as one of America's Top Green Cities. Located near the Blue Ridge Mountains, it is popular with outdoor enthusiasts with extensive opportunity for hiking, running, cycling, tennis, golf, and skiing.
The University of Virginia, including the UVA Health System and the University Physicians Group, is fundamentally committed to the diversity of our faculty and staff. We believe diversity is excellence expressing itself through every person's perspective and lived experiences. We are equal opportunity and affirmative action employers. All qualified applicants will receive consideration for employment.
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